A protein interaction map of Drosophila melanogaster.

نویسندگان

  • L Giot
  • J S Bader
  • C Brouwer
  • A Chaudhuri
  • B Kuang
  • Y Li
  • Y L Hao
  • C E Ooi
  • B Godwin
  • E Vitols
  • G Vijayadamodar
  • P Pochart
  • H Machineni
  • M Welsh
  • Y Kong
  • B Zerhusen
  • R Malcolm
  • Z Varrone
  • A Collis
  • M Minto
  • S Burgess
  • L McDaniel
  • E Stimpson
  • F Spriggs
  • J Williams
  • K Neurath
  • N Ioime
  • M Agee
  • E Voss
  • K Furtak
  • R Renzulli
  • N Aanensen
  • S Carrolla
  • E Bickelhaupt
  • Y Lazovatsky
  • A DaSilva
  • J Zhong
  • C A Stanyon
  • R L Finley
  • K P White
  • M Braverman
  • T Jarvie
  • S Gold
  • M Leach
  • J Knight
  • R A Shimkets
  • M P McKenna
  • J Chant
  • J M Rothberg
چکیده

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

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عنوان ژورنال:
  • Science

دوره 302 5651  شماره 

صفحات  -

تاریخ انتشار 2003